Cell Line Origin
Human Caucasian lung carcinoma
Cell Line Description
Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Application
Enzymatic studies
Human A549 cell line has been used:
- to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
- to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
- to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14
Culture Medium
Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human lung (carcinoma). growth mode: Adherent. karyotype: Hypotriploid. morphology: Epithelial. products: Lecithin, High amounts of de-saturated fatty acids.. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181908
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86012804-1VL
