Cell Line Origin
A9 tumour induced C3H mouse
Cell Line Description
This cell line was selected by passaging A9 (Sigma Catalogue number. 85011426) through syngenic C3H or semi allogenic mice over a period of three months and uptake was considerably higher than A9, thus A9 high uptake or A9HT. Karyotype is similar to A9 but with a slightly reduced total number of chromosomes, with the modal number of 53. This line lacks inosinic acid pyrophosphorylase.
Application
Tumourigenicity studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse unknown/unspecified. growth mode: Adherent. karyotype: Hyperdiploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51442305
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 88042804-1VL
- Temperature Control Device:
- Yes
