Abberior(R) STAR 580, NHS ester (C15-1303-141)

General description

Abberior STAR 580 is the latest development for STED microscopy with a fluorescent dye in the orange regime. The dye can be excited from 550 to 580 nm. Abberior STAR 580 can substitute dyes like Atto^™ 590/594 or AlexaFluor 584/594. The dye can most effectively be depleted in STED microscopy at 700 to 780 nm via e.g. a Ti:Sa laser or IR diodes.

Abberior STAR 580 is the dye of choice for orange fluorescence. Moreover, the dye is particularly designed and tested for 2-color STED microscopy in combination with Abberior STAR 635 using 2 separate STED wavelength. This offers the great advantage of using 2 regular (not featuring a long Stokes-shift) dyes, which are in general more photostable. Please see our 2-color dye section

Key features

• Exceptionally bright orange fluorescent dye
• Ideal for STED microscopy at 700-775 nm
• 2-color labeling partner with STAR 635P for 2-color STED microscopy

Absorption Maximum, λmax:: 587 nm (PBS, pH 7.4; water)
584 nm (MeOH, aq. ACN)
Extinction Coefficient, ε(λmax):85,000 M^-1cm^-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.17 (PBS, pH 7.4; water)
Correction Factor, CF280 = ε280/εmax: 0.15 (MeOH, aq. ACN)
Fluorescence Maximum, λfl:607 nm (PBS, pH 7.4)
604 nm (MeOH, aq. ACN)
Recommended STED Wavelength, λSTED:700 - 780 nm
Fluorescence Quantum Yield, η:0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.5 ns (PBS, pH 7.4)

Application

Anti-Mouse IgG-Abberior^® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections. Abberior^® STAR 580 conjugated with secondary antibody has been used for dual-color STED imaging of cultured hippocampal neurons.

Suitability

Designed and tested for fluorescent super-resolution microscopy

Other Notes

Find more infomation here Abberior^®

Legal Information

Atto is a trademark of Atto-Tec GmbH

abberior is a registered trademark of Abberior GmbH

Quality Level: 100. Assay: ≥. 70.0% (degree of coupling). solubility: DMF: 1 . mg/mL, clear. fluorescence: λ. _ex . 583 . nm. λ. _em . 605.0 . nm±10 . nm in PBS, pH 7. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.