Abberior(R) STAR 635, NHS ester (C15-1302-682)

General description

Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M^-1cm^-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)

Application

• Abberior^™ STAR 635 goat anti-rabbit antibody has been used for STED (stimulated emission depletion) imaging of primary cultured striatal neurons obtained from rats.
• Abberior^™ STAR 635 anti-rabbit antibody has been used for STED imaging of non-sensory supporting cells obtained from cochlea of rats and mice.
• Abberior^™ STAR 635 conjugated with secondary antibody has been used for STED imaging of microtubules in cells.

Suitability

Designed and tested for fluorescent super-resolution microscopy

Other Notes

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Legal Information

6538 is a trademark of American Type Culture Collection

abberior is a registered trademark of Abberior GmbH

Quality Level: 100. concentration: ≥. 50.0% (degree of coupling). solubility: DMF: 0.25 . mg/mL, clear. fluorescence: λ. _ex . 635 . nm. λ. _em . 655 . nm±10 . nm in PBS, pH 7.4. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.