ABTS SOLUTION

General description

ABTS^™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.

Application

ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.

Biochem/physiol Actions

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS^™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS^™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.

Physical form

Reaction product:
Color: green
Evaluation: photometric (405nm)

Analysis Note

Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ABTS is a trademark of Roche