AccuChIP Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control

General description

AccuChIP Chromatin Immunoprecipitation Internal Controls are designed to improve the accuracy of ChIP experiments. This simple to spike-in reagent is composed of a target-specific peptide sequence covalently bound to a uniquely designed double stranded nucleic acid sequence. Because AccuChIP controls mimic the behavior of cross-linked chromatin, by simply adding a known amount of the AccuChIP reagent to your chromatin and performing a standard ChIP reaction followed qPCR detection you can improve the accuracy and reproducibility of your ChIP experiments.

Features and Advantages

• Simple to use - just add to chromatin either before or after sonication
• Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals
• Target specific control - uses the same antibody used for your ChIP experiment
• Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling
• Easily incorporated into virtually any ChIP protocol
• Readily detected by qPCR
• Easily incorporated into ChIP-seq libraries
• Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity control

Application

Research Category
Epigenetics & Nuclear Function

Target-specific spike-in controls that make ChIP experiments more quantitative and accurate. Applications include:

• Normalization of ChIP signals
• Normalization of ChIP-seq signals
• Analysis of antibody specificity

Components

AccuChIP H3K27me2 Spike-In Reagent (50 pg/uL )

AccuChIP H3K27me2 Detection Primer Mix (5 μM)

AccuChIP Dilution Buffer

Physical form

One box containing three vials (one vial each of target specific AccuChIP Spike-In Reagent, target specific AccuChIP Detection Primer Mix, and AccuChIP Dilution Buffer)

Storage and Stability

AccuChIP Chromatin Immunoprecipitation Internal Controls are stable for 6 months at -20 ºC from date of receipt.
AccuChIP Spike In Reagent is provided as a 20X stock and should be diluted with the provided AccuChIP Dilution Buffer to create a working stock. Diluted AccuChIP Spike In Reagent can be stored in aliquots at -20 ºC for up to 3 months. AccuChIP Spike In reagent is composed of a peptide-double stranded DNA conjugate in a buffer containing BSA and glycerol to reduce adherence to the tube. The use of siliconized microfuge tubes for storage of diluted material is recommended.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.