ACCUSPIN(TM) Tubes Sterile, 12 mL Capacity (C15-1314-908)

General description

ACCUSPIN^™ Tubes are intended for use with the density gradient separation medium Histopaque^®-1077 in the isolation of lymphocytes and other mononuclear cells. The ACCUSPIN Tube is a specially designed polypropylene centrifuge tube with two chambers separated by a porous high-density polyethylene barrier (frit).

Application

ACCUSPIN^™ Tubes Sterile has been used in isolation of cord blood-derived endothelial progenitor cells. Accupusin separation tubes are also used to isolate peripheral mononuclear blood cells.

Principle

Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places the Histopaque-1077 below the frit. The blood sample can be added to the top chamber of the tube without the risk of mixing with the Histopaque-1077 in the lower chamber under the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the forgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes become lightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upper chamber or by means of a pipette. Erythrocytecontamination is avoided due to the barrier between thechambers.

Quantity

Package contains 20 tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC