Achromopeptidase from bacteria (C15-1315-104)

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀=0.6 over 2 hours at 37 °C.

Unit Definition

One unit will produce a change in A₆₀₀ of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Physical form

crude powder

form: lyophilized powder. Quality Level: 200. specific activity: ≥. 1,000 . units/mg solid. mol wt: 27 . kDa. application(s): diagnostic assay manufacturing. shipped in: dry ice. storage temp.: −. 20°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H334. Precautionary Statements: P261 - P342 + P311. Hazard Classifications: Resp. Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves.