Application
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.
Biochem/physiol Actions
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.
Unit Definition
One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).
Physical form
crude powder
form: lyophilized powder. Quality Level: 200. specific activity: ≥. 1,000 . units/mg solid. mol wt: 27 . kDa. application(s): diagnostic assay manufacturing. shipped in: dry ice. storage temp.: −. 20°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H334. Precautionary Statements: P261 - P342 + P311. Hazard Classifications: Resp. Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 12352204
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- A3547-500KU
- Temperature Control Device:
- Yes
