Adenosine Deaminase from bovine spleen (C15-1291-445)

General description

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.