Cell Line Origin
Horse inguinal lymph node x AFRC 17D1
Cell Line Description
The heterohybridoma AFRC 85D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79(H3N8)). For the establishment of AFRC 85D6 horse cells from the inguinal lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).
Immunogen
Influenza A equine 2 virus
Application
Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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biological source: Horse x Mouse/Horse Hybridoma. ImmunoDon: Horse inguinal lymph node. Myeloma: AFRC 17D1. Reactivity: Anti-Haemagglutinin, influenza. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51131610
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94120833-1VL
