Cell Line Origin
Mouse NS1 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against WC1 antigen found on bovine CD4- CD8- and CD2- T cells which express gamma/delta TcR. The antigen has a molecular weight of 215-300kDa and is equivalent to the T19 antigen in sheep. The antibody is directed against equivalent antigen in sheep and goats.
Immunogen
Bovine lymphocytes
Application
Flow cytometry, immunohistology, immunoprecipitation, complement lysis, in vivo depletion
Culture Medium
RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS1. Reactivity: Anti-WC1, bovine. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51204203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 88022410-1VL