Cell Line Origin
Rat YB2/3.0 Ag20 x Rat AO spleen
Cell Line Description
The antibody recognises glycoprotein 2BII. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. The antibody also cross-reacts with cell wall of Lobomonas piriformis. AFRC MAC 20 shows similar fine specificity and properties to AFRC MAC 18 and 19.
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Rat x Rat Hybridoma. ImmunoDon: AO rat spleen. Myeloma: YB2/3.0 Ag20. Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86030309-1VL