Cell Line Origin
Rat Y3Ag1.2.3 x Rat AO spleen
Cell Line Description
The antibody recognises the glycoprotein 2BII and labels both the whole cell surface and also the flagella. Binding is lost after acid hydrolysis or de-glycosylation of 2BII.
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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biological source: Rat x Rat Hybridoma. ImmunoDon: AO rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 12352202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85102265-1VL