Cell Line Origin
Rat Y3Ag1.2.3 x Rat AO spleen
Cell Line Description
The antibody recognises the glycoprotein 2BII of Chlamydononas reinhardii. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 7 shows similar specificity and properties to AFRC MAC 6, 8, 9, 10 and 11.
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
biological source: Rat x Rat Hybridoma. ImmunoDon: AO rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51282910
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85102269-1VL
