AGAR WASHED--PLANT CELL CULTURE TESTED (C005B-087798)

General description

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:

• as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth
• in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II
• as a component in the complete medium to regenerate protoplasts of Piriformospora indica
• as a clean agar medium to culture Synechococcus WH7803 strain

Biochem/physiol Actions

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Preparation Note

Washed