AGAROSE I 25G

Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility.  Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).   Specifications Moisture: ≤ 7% Ash: 4% EEO: 0.05-0.13 Sulfate: ≤ 0.1% Clarity 1.5% (NTU): ≤ 3 Gel Strength 1% (g/cm 2 ): ≥ 1200 Gel Strength 1.5% (g/cm 2 ): ≥ 2500 Gelling Temperature 1.5% (ºC): 36 ± 1.5 Melting Temperature 1.5% (ºC): 88 ± 1.5 DNase/ RNase Activity: None Detected DNA Resolution ≥1000bp: Finely resolved Gel background: Very low   Appropriate Agarose Concentrations GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER 0.6 1,000-20,000bp 1,000-15,000bp 0.8 500-12,000bp 500-10,000bp 1.0 300-8,000bp 250-7,000bp 1.2 200-6,000bp 200-5,000bp 1.5 100-3,500bp 100-3,000bp 2.0 50-2,000bp 50-2,000bp Features Extraordinary mechanical resistance for more reliable and easier handling Greater thermal stability due to high hysteresis (difference between gelling and melting temp) High transparency and visibility Easy preparation Applications Analytical nucleic acid electrophoresis Preparative nucleic acid electrophoresis Blotting Protein electrophoresis such as radial immunodiffusion