AGAROSE II LOW MELTING 25G

Agarose II has a low melting and gelling temperature compared to standard agaroses.  The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature.  The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice.    Specifications Moisture: ≤ 7% Ash: ≤ 0.4% EEO (electroendosmosis): ≤ 0.12 Sulfate: ≤ 0.12% Clarity 1.5% (NTU): ≤ 4 Gel Strength 1.5% (g/cm 2 ): ≥ 500 Gelling Temperature 1.5% (ºC): 24-28 Melting Temperature 1.5% (ºC): ≤ 65.5 DNase/ RNase Activity: None Detected DNA Resolution ≥1000bp: Finely resolved Gel background: Very low   Appropriate Agarose Concentrations GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER 0.75 500-20,000bp 500-12,000bp 1.00 300-16,000bp 300-8,000bp 1.25 250-10,000bp 200-4,000bp 1.50 200-5,000bp 150-3,000bp 1.75 100-2,500bp 100-2,000bp 2.0 50-1,500bp 50-1,000bp Features Lower gel strength compared to standard agarose Higher clarity Easy to handle Applications Electrophoresis of DNA Fragments >1000bp Tissue and Cell Culture Viral Plaque Assays