AGAROSE IV 25G

Agarose IV is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses.  This characteristic produces a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.  For improved resolution, we recommend storing the gel at 4°C for 30 minutes before use. Specifications: Moisture: ≤ 7% Ash: ≤ 0.25% EEO (Electroendosmosis): ≤ 0.12 Sulfate: ≤ 0.12% Clarity (NTU): ≤ 4 Gel Strength 1% (g/cm²): ≥ 1800 Gel Strength 1.5% (g/cm²): ≥ 3200 Gelling Temperature 3% (°C): ≤ 36±1.5 Melting Temperature 3% (°C): ≤ 88±1.5 DNase/ RNase Activity: None Detected Gel Background: Very Low  Appropriate Agarose Concentrations: Gel Concentration TAE Buffer [1X] 1.8% 200-5,000bp 1% 400-10,000bp 0.8% 1,000-15,000bp 0.5% 2,000-20,000bp 0.3% 3,000-40,000bp Migration in the TBE Buffer is slower, therefore lower concentrations can be used to obtain similar separation ranges. Applications: Conventional Electrophoresis Pulsed Field Gel Electrophoresis: Due to its higher exclusion limit, large molecules can be separated Blotting Agarose Bead Preparation Cell and Enzyme Immobilization