AGAROSE LE 100G

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose LE has been used for:

• analyzing fragments between 0.2 and 15 kb
• analysis of PCR products
• examination of restriction endonucleases
• digests of plasmid, cosmid, and λ phage DNA
• electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
• in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.

Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.