Aladdin™ EDC (C09-1510-600)

EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins. : :Characteristics of EDC:• Reactive group:carbodiimide:• Reaction target: activates carboxyl groups to conjugate to amino groups (primary amines):• Several conjugation strategies—react EDC alone with target groups or includecarbodiimide:• Reactivity: Forms active intermediate with carboxyl groups at pH 4.7–6.0 (optimum), then intermediate reacts with primary amines Specifications and Purity: Standard Molecular Formula: C8H18ClN3 Molecular Weight: 191.70 EC Number: 247-361-2 PubChem CID: 2723939 Isomeric SMILES: CCN=C=NCCCN(C)C.Cl Beilstein Registry Number: 5764110