Product introduction:The :Click-iT™ :O:-GlcNAc Enzymatic Labeling System: provides a highly sensitive and efficient method for the in-vitro modification of :O:-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to :O:-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT™ Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT™ glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.• :Green Features:Less hazardous :• :Labeling Target:Proteins(Glycoproteins)
- UPC:
- 12352204
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A638749-1kit
- Product Size:
- 1kit
