Aladdin™ Phosphine-PEG3-Biotin

Product introduction:EZ-Link Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.Features of EZ-Link Phosphine-PEG3-Biotin:• :Soluble:—easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples:• :Compatible:—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents:• :Chemoselective:—the phosphine reactive group is specific in biological samples for bioorthogonal azide-tagged molecules, ensuring that biotinylation is specific:• :PEG spacer:—polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein:When used in combination with azide labeling strategies, this compound enables detection or affinity purification of protein interactions and post-translational modifications using streptavidin probes or streptavidin agarose resins. The phosphine group of Phosphine-PEG3-Biotin conjugates to azide groups by theStaudinger reaction mechanism. Azide groups can be introduced into proteins or other cellular targets through :in vivo: labeling with azide-tagged derivatives of naturally occurring metabolic building blocks. Because neither phosphines nor azides are present in biological systems, they comprise a chemoselective (mutually specific) ligation pair for labeling and conjugation.• :Spacer:Extra-Long, Pegylated :• :Cell Permeability:Cell-Impermeant.