Aladdin™ Streptavidin Super Bright 780 Conjugate

Product introduction:eBioscience Streptavidin Super Bright 780 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.Applications tested:Streptavidin Super Bright 780 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 10:5: to 10:8: cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads.Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.Conjugate:Super Bright 780