Albumin, Human Serum, Non-denatured

General description

High purity plasma protein prepared under non-denaturing conditions.

Human serum albumin (HSA) is the most abundant protein present in the plasma. It is a monomeric multi-domain macromolecule comprising three homologous domains, numbered I, II, and III. Each domain consists of subdomains A and B with common structural motifs. The HSA gene is mapped to the human chromosome 4q13.3.

Application

Albumin, human serum, non-denatured has been used:

• to determine the contribution of albumin protein complexes to esterase activity in saliva,
• in tris buffer for monomer degradation studies and in protein adhesion assay
• to study the effect of Cu²⁺ and Zn²⁺ ions on human serum albumin interaction with plasma unsaturated fatty acids

Biochem/physiol Actions

Human serum albumin (HSA) functions as a carrier for fatty acids, steroids, and thyroid hormones. It is used as a supplement in cell culture medium to produce vaccines and pharmaceuticals. HSA is a potential biomarker for various diseases, like cancer, rheumatoid arthritis, ischemia, and post-menopausal obesity. It is also used to treat surgical blood loss, trauma, hemorrhage, and cardiopulmonary bypass. HSA is implicated in biological applications such as implantable biomaterials, surgical adhesives and sealants, and bio chromatography.

Warning

Toxicity: Standard Handling (A)

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Travis, J., and Pannell, R. 1973. Clin. Chem. Acta49, 49.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany