Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate

Product introduction:Alexa Fluor™ 488 streptavidin: comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:• :Alexa Fluor™ 488 streptavidin conjugate has Ex/Em maxima of ∼ (495/519):• :Bright, photostable fluorescence:• :High solubility in aqueous solutions:• :Available in multiple colors:• :Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more:Properties of Alexa Fluor™ Dyes:Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibitimproved photostability andbrightness and improved solubility in aqueous solutions. Available in abroad range of colors, these dyes are a good choice for most imaging applications.Blocking Endogenous Biotin:Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Learn more about Avidin-Biotin Detection:Learn more about Alexa Fluor™ Dyes:Find out about other Labeled Streptavidin Conjugates:Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook:• :Conjugate:Alexa Fluor™ 488, FluoroNanogold™