General description
Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.
Application
Alginate Lyase has been used:
- in the pre-processing of enzyme
- to de-encapsulate cells
- to digest Pseudomonas exopolysaccharide to facilitate electroporation
Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .
Biochem/physiol Actions
Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.
Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.
Unit Definition
One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C
Preparation Note
Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
- UPC:
- 51283933
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- A1603-100MG
- CAS:
- 9024-15-1
