Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse (C15-1315-434)

General description

A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.

Monoclonal Anti-Alkaline Phosphatase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse has been used in:

• Immunohistological analysis
• Immunohistochemistry assay
• Immunocytochemical staining

Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.

Biochem/physiol Actions

Mouse Monoclonal Alkaline Phosphatase Anti-Alkaline Phosphatase is a soluble complex formed from levamisole-resistant calf intestinal alkaline phosphatase and purified mouse monoclonal antibody to alkaline phosphatase. The use of a mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (APAAP) complex, in conjunction with a primary mouse antibody and a secondary bridging antibody (anti-mouse IgG), results in an intense signal with a very low background, while avoiding problems inherent to the covalent conjugation of antibodies. The technology of unlabelled antibody peroxidase-anti peroxidase (PAP) and the alkaline phosphatase anti- alkaline phosphatase (APAAP) method has applications in immunostaining. APAAP monoclonal antibodies complex are homogenous and this method is effective and its sensitive.

Physical form

Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: mouse. Quality Level: 200. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: AP1B9, monoclonal. description: Concentrate. form: buffered aqueous solution. technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil. isotype: IgG1. shipped in: wet ice. storage temp.: 2-8°C. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.