General description
Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.
Application
Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
- to analyse direct alkaline phosphatase (AP) activity
- to perform alkaline phosphatase assays with colorimetric system
- to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3
Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.
Physical form
A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
form: solution. Quality Level: 200. usage: sufficient for 400 . mL working substrate. storage temp.: 2-8°C. Storage Class Code: 10 - Combustible liquids. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB0300-1KT
