General description
The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.
Application
Alkaline Phosphatase Diethanolamine Activity Kit has been used:
- in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
- for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
- for determining ALP activity in the bone metastasis model of lung cancer
- for measuring ALP levels in colon tissue homogenates
Suitability
Suitable for the measurement of alkaline phosphatase activity in a variety of samples
Principle
Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 ï‚°C, the reaction is followed by monitoring the increase in absorbance at 405 nm.
usage: sufficient for 100 . tests. Quality Level: 200. storage temp.: 2-8°C. Pictograms: GHS05,GHS08. Signal Word: Danger. Hazard Statements: H315 - H318 - H361fd - H373. Precautionary Statements: P202 - P260 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313. Hazard Classifications: Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41141606
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AP0100-1KT
