General description
Consanguineous: Yes
Homozygous: Yes
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 217
Cw: 303
B: 1501
HLA DRA: 102
HLA DRB1: 1402
HLA DRB3: 101
HLA DQAP: 4.1
HLA DQA1: 503
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 402
Serological Profile
HLA A: 2
HLA A ief: 2.4
HLA C: 9
HLA B: 62
HLA B ief: 62.3
Bw4/bw6: 6
HLA Dw: 16
HLA DR: 14
HLA Dw2: 24
HLA DR52: 52
HLA DQ: 7
Culture Medium
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
biological source: human blood. ethnicity: Native South American, Warao. gender: female. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice.Shipping Information:
Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51201571
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- 88052064-1VL
- Temperature Control Device:
- Yes
