Amicon® Ultra Centrifugal Filter, 3 kDa MWCO

Description General description Amicon® Ultra 15 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >90% . The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times. We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Application Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane. Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS. Concentration of dilute or pre-purified proteins from column eluents. Desalting, buffer exchange and protein dialysis. Features and Benefits Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%. Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes). Heat-sealed membrane minimizes downstream extractables. 100% integrity tested for reliable performance. Convenient sample monitoring with translucent housing and volume gradations. Direct pipettor sample access eliminates processing step to recover concentrate. High concentration factors of 80–100X. Physical form Color Code: Clear with pink cap Legal Information Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Q1: What is the molecular weight cutoff (MWCO) of this Amicon® Ultra filter? A: The filter has a 3,000 Dalton MWCO, ideal for retaining proteins and macromolecules larger than 3 kDa. Q2: What is the sample recovery rate? A: The filter achieves greater than 90% recovery for dilute samples, ensuring minimal protein loss. Q3: What types of samples can be processed? A: It is suitable for antigens, antibodies, enzymes, nucleic acids, and protein samples from tissue extracts or lysates. Q4: What is the capacity and design benefit? A: It can handle up to 15 mL, and the vertical membrane design minimizes fouling and speeds up filtration (as fast as 10–15 minutes). Q5: Is this product environmentally friendly? A: Yes, the inner filtration device is made from 60% bio-circular material using the ISCC mass balance approach.