Application
Aminopterin concentrate supplement for hybridoma cell culture applications.
Biochem/physiol Actions
Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.
Linkage
More potent, but more toxic, than methotrexate.
Reconstitution
Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 μM aminopterin.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
- UPC:
- 51111664
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A5159-10VL
