Ampicillin Dextrin Agar Base, 2.5 kg

Ampicillin Dextrin Agar Base is used for differential and selective isolation of Aeromonas species from water samples using membrane filter technique. It differentiates gram negative rods such as Pseudomonas and Enterobacteriaceae from Aeromonas. Aeromonas ferments dextrin and acid produced is indicated by color change from blue to yellow by the pH indicator, bromothymol blue. Ampicillin enhances selectivity of the medium. Aeromonas exhibit positive Nadi reaction (dextrin degradation) is indicated by a purple color at the periphery of the colony. Typical Aeromonas species appear as large, convex yellow colonies with a purple periphery. Appearance: Light yellow to greenish yellow homogeneous free flowing powder Gelling: Firm, comparable with 1.5% Agar gel Color and Clarity of prepared medium: Dark green colored clear to slightly opalescent gel forms in Petri plates Reaction of 3.74% w/v aqueous solution at 25°C. pH : 8.0±0.1 Cultural characteristics observed with added Ampicillin Dextrin Selective Supplement (FD107A), after an incubation at 35-37°C for 18-24 hours