AMPK (alpha1/beta1/gamma1), His.Tag, Human, Recombinant, S. frugiperda

General description

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening.

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1).

M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)

Warning

Toxicity: Standard Handling (A)

Physical form

In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Scott J.W., 2007. EMBO J26, 806.
Minokoshi Y et al. 2004. Nature428, 569.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany