General description
Full length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
Kinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
Physical form
In 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C).
Other Notes
Foretz, M., et al. 2005. Diabetes54, 1331.
Viollet, B., et al. 2003. Biochem. Soc. Trans.31, 216.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 171539-5UG