Anaerobic Blood Agar Base, 5KG

Anaerobic Blood Agar Base is recommended for cultivation of anaerobic microorganisms, including very fastidious organisms from clinical specimens. Anaerobic Blood Agar base serves as a nutritious, nonselective medium allowing the cultivation of not only fastidious anaerobes but also of aerobic and microaerophillic microorganisms. It promotes both typical pigment formation in Bacteroides melaningenicus and displays double haemolytic reaction in Clostridium perfringens with added blood to the medium base. The inner zone of haemolysis is due to toxin and the outer zone of incomplete haemolysis to toxin (lecithinase activity). Appearance: Yellow to tan colored homogeneous free flowing powder Basal medium : Yellow colored; with addition of 5% v/v sterile, defibrinated sheep blood : cherry red colored Basal medium : slightly opalescent; After addition of 5% v/v sterile, defibrinated sheep blood : opaque gel in petri plates Reaction of 4.4% w/v aqueous solution at 25°C. pH : 7.4±0.2 Cultural characteristics observed after 24-48 hours at 35-37°C with 5-10% CO2