Angiotensin I Converting Enzyme (ACE) Activity Assay Kit (Fluorometric)

General description

The Angiotensin I Converting Enzyme (ACE) Activity Assay is based on the cleavage of a synthetic fluorogenic peptide. The measured fluorescence is directly proportional to the ACE activity present. The reaction is summarized as follows: ACE ACE Fluorogenic substrate ———————> Cleaved ACE Substrate + fluorescent product (λ_ex 320nm/λ_em 405nm)

The ACE Positive Control in the kit can be used to screen for ACE inhibitors. This kit has a linear range of 1.5-110 mU of ACE activity.

Angiotensin Converting Enzyme (ACE) is a key component in the renin-angiotensin system (RAS), which is well known for its role in the regulation of blood pressure, electrolyte balance, and vascular remodeling^1,2 . ACE is located mainly in the capillaries of the lungs, but can also be found in endothelial and kidney epithelial cells³. This hormone system also regulates body fluids volume. Renin produces angiotensin I by cleaving several amino acids from angiotensinogen. ACE then hydrolyzes angiotensin I to yield active angiotensin II. Angiotensin II also stimulates the adrenal cortex to secrete aldosterone, which also causes an increase in blood pressure by stimulating sodium reabsorption by the kidney^1. Since ACE exhibits critical functions regulating RAS, ACE inhibition has become a promising approach for drug targeting in the treatment of cardiovascular diseases such as hypertension, heart failure, and diabetic nephropathy.

The detection range : 1.5-110 mU of ACE activity.

Application

• Detection of ACE1 activity in Tissue/Cell lysates, Serum, Plasma
• Enables to screen for ACE inhibitors

Features and Benefits

Our economical ACE Kit also saves your time and efforts:

• A simple, quick, sensitive, and direct procedure for measuring ACE levels in a variety of samples such as serum and plasma
• Enables to screen for ACE inhibitors
• Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
• User friendly: No need to weigh or mix multiple reagents
• Quick instruction bench card – to assure your experimental success
• More experiments in one kit - contains sufficient reagents for 200 tests.

Unit Definition

One unit of ACE is defined as the amount of enzyme that releases 1 nmol of fluorescent product from the substrate, in 1 minute, under the assay conditions, at 37 °C.This kit contains sufficient reagents for 200 tests.