ANNEXIN V APOPTOSIS, 20 ASSAYS

Apoptosis is a form of programmed cell death which is essential as a normal part of development in multicellular organisms. Apoptosis go awry in some disease states such as Alzheimer’s and cancer. Annexin V-FITC Apoptosis Assay is designed to detect apoptosis. It is based on a property of apoptotic cell where during onset of apoptosis, cells translocate the membrane phosphatidyl serine from inner face of plasma membrane to cell surface. Annexin V protein has high binding affinity for phosphatidyl serine in presence of calcium ions. Thus Annexin V-FITC bound cell is apoptotic and can be quantified by FACS. Furthermore, propidium iodide is supplied in kit to differentiate apoptotic cells from necrotic cells. Features: Designed to detect cells undergoing apoptosis Available in 100 assays or 500 assays formatwith 100 μl assay volume. Can differentiate between apoptotic and dead cells. Suitable for flowcytometery and fluorescence microscope based experiments. Rapid :Staining can be done within 30minutes. Advantage(s): detect cells undergoing apoptosis Can differentiate between apoptotic cells and necrotic cells as necrotic cells are stained with propidium iodide.