General description
Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s.
The 14-3-3 gamma antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.
Specificity
14-3-3 gamma. By Western blot the antibody recognizes the ~30 kDa protein.
Immunogen
Synthetic peptide from the N-terminal area of sheep 14-3-3 gamma.
Application
Anti-14-3-3 γ Antibody detects level of 14-3-3 γ & has been published & validated for use in ELISA, WB, IH.
Western blot: 1:6,000-1:12,000 using ECL.
Immunohistochemistry: 1:400-1:800 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA
Optimal working dilutions must be determined by end user.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, rat, sheep, chicken, mouse, bovine. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_012479.2. UniProt accession no.: P61981. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... YWHAG(7532). Storage Class Code: 10 - Combustible liquids.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51172414
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- AB9738
- Temperature Control Device:
- Yes
