ANTI-8-OXOGUANINE CL 483.15 (ASC.FREE)

General description

8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer′s and Parkinson′s.

Specificity

8-oxoguanine. By competitive ELISA the monoclonal showed no reactivity with dGMP, dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range.

Target DNA modification is not species-specific.

Immunogen

8-oxoguanine adsorbed on to alumina.

Application

Immunohistochemistry Analysis: A representative lot detected cells with 8-oxoguanine (8-oxoG) immunoreactivity in the central veins (CV) and portal triads (PT) regions of paraffin-embedded kettle liver sections (García-Vaquero, M., et al. (2012). Animal.6(9):1435-1443).
Immunohistochemistry Analysis: A representative lot detected increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in paraffin-embedded coronal hippocampus sections from mice induced to express mutated mitochondrial isoform Uracil–DNA glycosylase by fluorescent immunohistochemistry (Lauritzen, K.H., et al. (2011). DNA Repair (Amst). 10(6):639-653).
Immunohistochemistry Analysis: A representative lot detected significantly increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in ovaries excised from neonatal mice upon exposure to ovotoxic agent 4-vinylcyclohexene diepoxide (VCD), methoxychlor (MXC), or menadione (MEN) in culture by fluorescent immunohistochemistry (Sobinoff, A.P., et al. (2010). Toxicol. Sci. 118(2):653-666).
Immunocytochemistry Analysis: A representative lot detected significantly increased 8-oxoguanine (8-oxoG) immunoreactivity in RINm5F rat insulinoma cells upon IL-1beta or ROS-inducer menadione treatment (Mehmeti, I., et al. (2011). Biochim. Biophys. Acta. 1813(10):1827-1835).
Immunocytochemistry Analysis: A representative lot detected significantly increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in AC16 human cardiomyocytes upon infection by the hemoflagellate protozoan parasite Trypanosoma cruzi (Ba, X., et al. (2010). J. Biol. Chem. 285(15):11596-11606).
Immunocytochemistry Analysis: Representative lots detected nutrient-deprivation-induced enhancement of nuclear 8-oxoguanine (8-oxoG) immunoreactivity in HeLa and COS-7 cells by fluorescent immunocytochemistry (Conlon, K.A., et al. (2003). DNA Repair (Amst). 2(12):1337-1352; Conlon, K.A., et al. (2000). J. Histotechnol. 23(1):37-44).
ELISA Analysis: Specificity of a representative lot against 8-oxoguanine was confirmed by competitive ELISA. Competition by dGMP, dAMP, dCMP or TMP was only observed when the concentrations were increased to the millimolar range.

This Anti-8-Oxoguanine, clone 483.15 Antibody (Ascites Free) is validated for use in Immunocytochemistry, Immunohistochemistry and ELISA for the detection of 8-Oxoguanine.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected 8-Oxoguanine in HeLa cells.

Linkage

Replaces: MAB3560

Physical form

Format: Purified

Purified mouse monoclonal IgMκ antibody in PBS without preservatives (azide free).

Other Notes

Concentration: Please refer to lot specific datasheet.

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