ANTI-A-GALACTOSIDASE A. CLONE 6G8 (C005B-449035)

General description

Bone morphogenetic protein 10 (UniProt: O95393; also known as BMP-10) is encoded by the BMP10 gene (Gene ID: 27302) in human. BMP-10 is a disulfide-linked homodimeric protein that is synthesized with a signal peptide (aa 1-21) and a propeptide (aa 22-316) that are subsequently cleaved off to generate the mature form (aa 317-424). It can act as a homodimer or can heterodimerize with BMP-9 to serve as a high affinity ligand for endothelial receptor activin-receptor-like kinase 1 (ALK1) that plays a key role in vascular remodeling. Both BMP-9 and 10 are shown to be present in blood and their levels are elevated in mice around birth, which suggests that they play an active role in pre- and postnatal development. They are reported to be essential for proper closure of the ductus arteriosus. BMP-10 is also essential for maintaining the proliferative activity of embryonic cardiomyocytes. It prevents the premature activation of the negative cell cycle regulator CDKN1C/p57KIP. BMP-9 and 10 share about 65% sequence identity and studies with either BMP-9 or BMP-10 knockout mice have shown that plasma from these KO mice lack the ability to activate ALK-1-transfected 3T3 cells, which suggests that most of the biological activity resides in the circulating heterodimeric form. In mammals, the major organ for BMP-9 production is the liver and BMP-10 is produced in the cardiac tissue. (Ref.: Tillet, E., et al. (2018). J. Biol. Chem. 293(28); 10963-10974; Levet, S., et al. (2015). Proc. Natl. Acad. Sci. USA. 112(25); E3207-3215).

Specificity

Clone 13C11 is a mouse monoclonal antibody that detects human Bone morphogenic protein 10 (BMP-10).

Immunogen

His-tagged full-length recombinant human Bone morphogenetic protein 10 (BMP-10).

Application

Quality Control Testing

Evaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected BMP-10 in Human heart tissue lysate.

Tested Applications

Neutralizing Analysis: A representative lot of this antibody neutralized BMP-10 and exacerbated the remodeling defect in BMP-9 knockout mice pups and led to a reopening of the ductus arteriosus. (Levet, S., et. al. (2015). PNAS. 112(25):E3207-15)

Western Blotting Analysis: A representative lot detected BMP-10 in Western Blotting applications (Tillet, E., et. al. (2018). J Biol Chem. 293(28):10963-10974).

ELISA Analysis: A representative lot detected BMP-10 in ELISA applications (Tillet, E., et. al. (2018). J Biol Chem. 293(28):10963-10974).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-BMP-10, clone 13C11, Cat. No. MABS1936, is a mouse monoclonal antibody that detects Bone morphogenetic protein 10 (BMP-10) and is tested for use in ELISA, Neutralizing, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.