ANTI-ABBA ANTIBODY (C15-1315-956)

General description

Radial glia play key roles in neuronal migration, axon guidance, and neurogenesis during development of the central nervous system. However, the molecular mechanisms regulating growth and morphology of these extended cells are unknown. ABBA, a novel member of the IRSp53/MIM protein family, is enriched in different types of radial glia. ABBA binds ATP-actin monomers with high affinity and deforms PIP2-rich membranes in vitro through its WH2 and IMdomains, respectively. In radial glia-like C6-R cells, ABBA localizes to the interface between the actin cytoskeleton and plasma membrane, and its depletion by RNAi leads to defects in lamellipodial dynamics and process extension. Together, ABBA acts as a novel regulator of actin and plasma membrane dynamics in radial glial cells, and provides evidence that the membrane binding/deformation activity is critical for the cellular functions of IRSp53/MIM/ABBA family proteins

Specificity

Cat. # AB2209 recognizes ABBA

May react with Pan troglodytes and Macaca mulatta based on sequence homology. Reactivity with other species was not tested.

Immunogen

Recombinant protein.

Application

Anti-ABBA Antibody is an antibody against ABBA for use in WB, IC, IH.

Non-Lot Specific Tested Application 1:
Immunocytochemistry: 1:50 dilution in mouse primary glial cell line and C6, C6-R rat cell line.

Non-Lot Specific Tested Application 2:
Immunohistochemistry: 1:1,000 in embryonic and adult brain tissue.

(See Reference for more information)

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Quality

Routinely tested on mouse brain lysate
Lot Specific Tested Application 1: Western Blot

Target description

~82 kDa

Physical form

Serum with with 0.05% NaN3

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.

Analysis Note

Control
Mouse brain lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, rat, mouse. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_941027. UniProt accession no.: Q6P9S0. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... MTSS2(92154). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.