Anti-acetyl Histone H3 Antibody (Lys 64) (C15-1316-852)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Histone H3 is reported to be acetylated at Lys64 (H3K64ac) by p300 co-activator. In addition, H3K64ac is found enriched at the transcriptional start sites of active genes, consistent with a transcriptional activation modification opposite of its repressive counterpart H3K64me3 (PMID 24668167). The target specificity of ABE1057 is confirmed with peptide-based epitope analysis by dot blot, using Cat. No. 16-667, AbSurance Histone H3 Antibody Specificity Array, and Cat. No. 16-665, AbSurance Histone H2A, H2B, H4 Antibody Specificity Array. Sequence surrounding the target acetylation site is conserved among human Histone H3 subtypes H3.1, H3.2, H3.3, and H3.3C/H3.5 (UniProt P68431, Q71DI3, P84243, and Q6NXT2, respectively).

Immunogen

Epitope: Acetylated Lys 64

KLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys 64).

Application

Anti-acetyl Histone H3 Antibody is an antibody against acetyl Histone H3 for use in Western Blotting, Immunocytochemistry, Dot Blot.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected acetyl Histone H3 (Lys 64) in HeLa, A431, and NIH/3T3 cells.
Dot Blot Specificity Analysis: Histone peptides with various modifications were probed with acetyl Histone H3 (Lys 64) using a 1:250 dilution.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected acetyl Histone H3 (Lys 64) in 10 µg of sodium butyrate treated HeLa cell lysate.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. technique(s): dot blot: suitable, immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_003520. UniProt accession no.: P68431. shipped in: wet ice. target post-translational modification: acetylation (Lys 64). Gene Information: human ... HIST1H3F(8968). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.