Anti-acetyl Histone H3 Antibody (Lys122) (C15-1316-844)

General description

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

Immunogen

Epitope: Near C-terminal

KLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys122) near the C-terminal.

Application

Anti-acetyl Histone H3 (Lys122) is an antibody against acetyl Histone H3 (Lys122) for use in WB, ICC, DB, IP.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected acetyl Histone H3 (Lys122) in HeLa and A431cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl Histone H3 (Lys122) in Histone peptides with various modifications.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated acetyl Histone H3 (Lys122) in 500 µg of sodium butyrate treated HeLa acid extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected acetyl Histone H3 (Lys122) in 10 µg of sodium butyrate treated HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human. technique(s): dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_003520. UniProt accession no.: P68431. shipped in: wet ice. target post-translational modification: acetylation (pLys122). Gene Information: human ... HIST1H3F(8968). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.