General description

Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.

Immunogen

synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.

Application

Anti-acetyl-Histone H4 [Ac-Lys¹²] antibody produced in rabbit has been used in:

immunoblotting
immunofluorescence
immunohistochemistry

Biochem/physiol Actions

Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys¹² (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.