General description
Histone H4 (UniProt P02309) is encoded by the HHF1 (ORF YBR0122; Gene ID 852294) and HHF2 (ORF N2752; Gene ID 855701) genes in yeast species. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Specificity
Broad species cross-reactivity is expected.
H4K16ac specificity of the unconjugated antibody (Cat. No. 07-329) has been confirmed using the Absurance
Immunogen
Epitope: H4K16ac
Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 (Lys16) in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.
This Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.
Target description
~10 kDa observed
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
- UPC:
- 51472702
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 07-329-AF488
- Product Size:
- 329-AF488
