ANTI-ACETYL (LYS26) PHOSPHO (SER27) HISTONE H1.4 ANTIBODY

General description

H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.

Specificity

Other homologies: Rat and Mouse (82% sequence homology).

This antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.

Application

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.

Dot Blot Analysis: A 1:1,320 dilution from representative lot detected acetyl (Lys26) phospho (Ser27) Histone H1.4 peptides.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Evaluated by Western Blot in colcemid treated HeLa acid extract.

Western Blot Analysis: A 1:3,000 dilution of this antibody detected acetyl (Lys26) phospho (Ser27) Histone H1.4 in 10 µg of colcemid treated HeLa acid extract.

Target description

~35 kDa observed. Uncharacterized bands may be observed at ~70 kDa and ~17 kDa in some acid extracts.

Physical form

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Colcemid treated HeLa acid extract

Disclaimer

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