Anti-Acetyl Lysine Antibody (C15-1316-140)

General description

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

Specificity

Recognizes proteins acetylated on lysine residues. This antibody recognizes multiple acetylated proteins including acetylated histones, acetylated BSA, and acetylated MBP, and exhibits no reaction with non-acetylated proteins.

Immunogen

Acetylated KLH.

Application

Anti-Acetyl Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Acetyl Lysine & has been validated in ELISA, IF, IHC, IP & WB.

Immunoprecipitation:
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.

Immunohistochemistry:
2.5 μg/mL of a previous lot was used.

Immunofluorescence:
5 μg/mL of a previous lot was used.

Optimal working dilutions must be determined by end user.

Research Category
Signaling

Epigenetics & Nuclear Function

Research Sub Category
General Post-translation Modification

Histones

Quality

Routinely evaluated by Western Blot on Hela Acid Extract/Sodium Butyrate lysates.

Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing PBS with 50% glycerol.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity (predicted by homology): all. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. shipped in: wet ice. target post-translational modification: acetylation (Lys). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.