ANTI-ACHAETE SCUTE HOMOLOG 2 ANTIBODY CLONE 8F1

General description

Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).

Specificity

This antibody recognizes Achaete Scute homolog 2.

Immunogen

His-tagged recombinant protein corresponding to mouse Achaete Scute homolog 2.

Application

Anti-Achaete Scute homolog 2 Antibody, clone 8F1 is an antibody against Achaete Scute homolog 2 for use in WB, IH.

Immunohistochemistry: A previous lot of this antibody was used in immunocytochemistry by an independent laboratory (Van der Flier, et al., 2009).

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 on 10 µg of human placenta tissue lysate.

Target description

~ 20 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.